2J4T
Biological and Structural Features of Murine Angiogenin-4, an Angiogenic Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.472, 31.422, 95.471 |
Unit cell angles | 90.00, 118.85, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.020 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bwk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.420 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.140 | 0.520 |
Number of reflections | 17076 | |
<I/σ(I)> | 11.3 | 2.8 |
Completeness [%] | 74.2 | 49.1 |
Redundancy | 2.08 | 0.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | INITIAL, TWINNED CRYSTALS WERE GROWN BY MIXING EQUAL VOLUMES OF PROTEIN SOLUTION (8 MG ML IN WATER) AND RESERVOIR (25 % PEG 3350, 0.2 M LI2SO4, 0.1 M TRIS-HCL, PH 8.5). THESE CRYSTALS WERE USED TO SEED PREEQUILIBRATED DROPS CONTAINING 4 MG ML PROTEIN, 20 % PEG 3350, 0.05 M LI2SO4, 0.09 M TRIS-HCL, PH 8.5. |