2J4H
Crystal structure of a H121A Escherichia coli dCTP deaminase mutant enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-11 |
Detector | MARRESEARCH |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 62.545, 62.545, 342.444 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.27300 |
Structure solution method | OTHER |
RMSD bond length | 0.013 |
RMSD bond angle | 1.579 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 0.470 |
Number of reflections | 11131 | |
<I/σ(I)> | 4.9 | 1.3 |
Completeness [%] | 94.2 | 75.1 |
Redundancy | 10 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.8 | VAPOUR DIFFUSION, 34% PEG400, 0.2M MAGNESIUM CHLORIDE, 0.1M HEPES PH6.8, 5MM DCTP |