2J3K
Crystal structure of Arabidopsis thaliana Double Bond Reductase (AT5G16970)-Ternary Complex II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 277 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.080, 122.450, 147.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.199 |
Rwork | 0.199 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.025 |
RMSD bond angle | 4.600 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.050 |
Number of reflections | 23815 |
Completeness [%] | 82.6 |
Redundancy | 5.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | reservoir solution containing 20% (w/v) polyethylene glycol 3350 and 0.2 M potassium chloride |