2J3E

Dimerization is important for the GTPase activity of chloroplast translocon components atToc33 and psToc159

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL17B2
Synchrotron site	NSRRC
Beamline	BL17B2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	71.521, 71.521, 106.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.170 - 3.200
R-factor	0.228
Rwork	0.228
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h65
RMSD bond length	0.010
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	3.200	3.200
Rmerge	0.100	0.370
Number of reflections	8739
<I/σ(I)>	11.2	2.9
Completeness [%]	98.5	98.7
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1