2J2M
Crystal Structure Analysis of Catalase from Exiguobacterium oxidotolerans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-07 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.267, 131.935, 110.642 |
Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8cat |
RMSD bond length | 0.013 |
RMSD bond angle | 1.430 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.050 | 0.060 |
Number of reflections | 99632 | |
<I/σ(I)> | 11.6 | 10.4 |
Completeness [%] | 99.0 | 97.8 |
Redundancy | 3.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 100MM MES PH6.0, 12-13%(W/V)PEG10000, 5%(W/V)PEG400 AND 20%(W/V)GLYCEROL, pH 6.00 |