2J2C

Crystal structure of Human Cytosolic 5'-Nucleotidase II (NT5C2, cN-II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-22
Detector	ADSC CCD
Spacegroup name	I 2 2 2
Unit cell lengths	91.465, 128.034, 130.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.200
R-factor	0.154
Rwork	0.152
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bde
RMSD bond length	0.016
RMSD bond angle	1.428
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.290	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.150
Number of reflections	39212
<I/σ(I)>	22.5	11.5
Completeness [%]	100.0	100
Redundancy	6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.8 M MG2SO4 0.1 M TRIS PH 8.5