2J20
Human p53 core domain mutant M133L-V203A-N239Y-N268D-R273C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.720, 70.979, 105.184 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.400 - 1.800 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bim |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.400 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.200 |
Number of reflections | 44922 | |
<I/σ(I)> | 22.5 | 6.1 |
Completeness [%] | 98.5 | 95.4 |
Redundancy | 7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |