2J1T

Structure of a Streptococcus pneumoniae fucose binding module in complex with the Lewis Y antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	113
Spacegroup name	P 21 21 21
Unit cell lengths	43.707, 59.943, 98.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.300 - 1.600
R-factor	0.151
Rwork	0.148
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.685
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.350
Number of reflections	34122
<I/σ(I)>	13.2	3.5
Completeness [%]	97.4	97.6
Redundancy	4.2	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1