2J1E

High Resolution Crystal Structure of CBM32 from a N-acetyl-beta- hexosaminidase in complex with lacNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	113
Spacegroup name	P 43 21 2
Unit cell lengths	77.377, 77.377, 73.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.179
Rwork	0.175
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.696
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Rmerge	0.100
Number of reflections	9130
<I/σ(I)>	8.6
Completeness [%]	99.6
Redundancy	4.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1