2J17
pTyr bound form of SDP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.661, 61.661, 217.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.340 - 2.840 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j16 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.223 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.660 | 2.990 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 10632 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 94.7 | 86.5 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 28% PEG 400, 0.1M HEPES, PH 7.5, 0.2M CACL2, 7MG/ML PROTEIN, 15MM PHOSPHOTYROSINE, 1:1 SITTING DROP VAPOUR DIFFUSION |
