2J16

Apo & Sulphate bound forms of SDP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-01
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	77.342, 77.342, 152.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.770 - 2.700
R-factor	0.232
Rwork	0.230
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ENSEMBLE MODEL
RMSD bond length	0.007
RMSD bond angle	1.196
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.060	0.370
Number of reflections	13396
Completeness [%]	99.7	99.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			1.5M MGSO4, 0.1M HEPES, PH 7.5 7MG/ML PROTEIN 1:1 SITTING DROP VAPOUR DIFFUSION