2J0N
A proteolytically truncated form of Shigella Flexneri IpaD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.790, 91.360, 54.910 |
| Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 24238 | |
| <I/σ(I)> | 7.9 | 1.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10 % (V/V) PEG 400, 0.1 M NACL, 0.1 M TRIS-HCL, PH7.0, pH 7.00 |
