2IZY

Molecular Basis of AKAP Specificity for PKA Regulatory Subunits

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-08
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	91.490, 91.490, 189.047
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.580 - 2.200
R-factor	0.22
Rwork	0.215
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.253
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.240
Number of reflections	23415
<I/σ(I)>	11.6
Completeness [%]	100.0	88.2
Redundancy	28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8		8-10% PEG400, 0.2-0.4 M SODIUM PHOSPHATE AND SODIUM CITRATE (PH 5.8)