2IZY
Molecular Basis of AKAP Specificity for PKA Regulatory Subunits
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 91.490, 91.490, 189.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.580 - 2.200 |
| R-factor | 0.22 |
| Rwork | 0.215 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.253 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 23415 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 100.0 | 88.2 |
| Redundancy | 28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 8-10% PEG400, 0.2-0.4 M SODIUM PHOSPHATE AND SODIUM CITRATE (PH 5.8) |
