2IZF

STREPTAVIDIN-BIOTIN PH 4.0 I222 COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	I 2 2 2
Unit cell lengths	95.000, 105.340, 47.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.580
R-factor	0.165
Rwork	0.165
R-free	0.20000
RMSD bond length	0.018
RMSD bond angle	27.200 *
Data reduction software	bioteX
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.580 *
Rmerge	0.044
Total number of observations	91953 *
Number of reflections	28476
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	30-32 (%sat)
2	1	reservoir	potassium acetate	0.1 (M)
3	1	drop	protein	30 (mg/ml)