2IYT
Shikimate kinase from Mycobacterium tuberculosis in unliganded state, open LID (conf. A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 44.192, 44.192, 152.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.470 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iys |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.766 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 1.510 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.100 | 0.720 |
| Number of reflections | 26142 | |
| <I/σ(I)> | 7.15 | 2.04 |
| Completeness [%] | 97.6 | 98.7 |
| Redundancy | 4.05 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 170MM NA ACETATE, 15%(V/V) GLYCEROL, 25.5%(W/V) PEG 4000, 85MM TRIS-HCL PH 8.5 |
