2IYG
Dark state structure of the BLUF domain of the rhodobacterial protein AppA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 127.710, 127.710, 65.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.990 - 2.300 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.23300 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.473 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.060 | 0.260 |
Number of reflections | 17447 | |
<I/σ(I)> | 15.4 | 5.5 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.50 |