2IY2
Crystal structure of the N-terminal dimer domain of E.coli DsbG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-10-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 56.842, 56.842, 42.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.790 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v58 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.310 |
| Number of reflections | 10806 | |
| <I/σ(I)> | 9.3 | 3.3 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M CADMIUM CHLORIDE, 33% MPD, pH 7.50 |
