2IXU
Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (wild-type endolysin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-14 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.521, 96.504, 127.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.320 - 2.280 |
| R-factor | 0.21236 |
| Rwork | 0.212 |
| R-free | 0.26238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h09 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.566 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.860 | 2.410 |
| High resolution limit [Å] | 2.280 | 2.260 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 32678 | |
| <I/σ(I)> | 6.6 | 1.8 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 9.3 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
