2IXP
Crystal structure of the Pp2A phosphatase activator Ypa1 PTPA1 in complex with model substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 86.886, 86.886, 410.626 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.120 - 2.800 |
R-factor | 0.233 |
Rwork | 0.229 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ixo |
RMSD bond length | 0.026 |
RMSD bond angle | 2.466 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.200 |
Number of reflections | 42938 |
<I/σ(I)> | 5.7 |
Completeness [%] | 99.9 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.8MM PEPTIDE, 1M AMMONIUM SULFATE, 3% GLYCEROL, 0.1M ADA PH 6.5 |