2IXP
Crystal structure of the Pp2A phosphatase activator Ypa1 PTPA1 in complex with model substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.886, 86.886, 410.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.120 - 2.800 |
| R-factor | 0.233 |
| Rwork | 0.229 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ixo |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.466 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.200 |
| Number of reflections | 42938 |
| <I/σ(I)> | 5.7 |
| Completeness [%] | 99.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.8MM PEPTIDE, 1M AMMONIUM SULFATE, 3% GLYCEROL, 0.1M ADA PH 6.5 |
