2IX4
Arabidopsis thaliana mitochondrial beta-ketoacyl ACP synthase hexanoic acid complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.109, 92.942, 74.217 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 71.070 - 1.950 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.934 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.100 | 2.030 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 64327 | |
| <I/σ(I)> | 4.8 | 1.8 |
| Completeness [%] | 95.6 | 70.5 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES-BUFFER PH 6.5, 12% PEG20000, 20 MG/ML HEXANOYL-COA |
