2IX3

Structure of yeast Elongation Factor 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	97.610, 110.660, 212.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.630 - 2.700
R-factor	0.216
Rwork	0.216
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.400
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.390
Number of reflections	63809
<I/σ(I)>	17.88	5.7
Completeness [%]	99.7	100
Redundancy	7.39	7.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.2		pH 5.20