2IX2
Crystal structure of the heterotrimeric PCNA from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.808, 78.798, 125.611 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 48.620 - 2.200 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ud9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 43983 | |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 91.0 | 93 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
