2IWV
Structure of the monomeric outer membrane porin OmpG in the open and closed conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-12 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 70.600, 77.000, 103.900 |
Unit cell angles | 79.30, 73.40, 74.30 |
Refinement procedure
Resolution | 14.980 - 2.300 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.26900 |
Structure solution method | OTHER |
RMSD bond length | 0.020 |
RMSD bond angle | 2.504 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.100 |
Number of reflections | 240777 |
<I/σ(I)> | 7.56 |
Completeness [%] | 90.6 |
Redundancy | 2.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.9 | 100 MM HEPES PH 7.5 30% PEG 4000 200 MM CACL2 |