2IWQ

7th PDZ domain of Multiple PDZ Domain Protein MPDZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-09
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	48.370, 48.370, 75.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.760 - 1.800
R-factor	0.205
Rwork	0.201
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i92 1g90
RMSD bond length	0.014
RMSD bond angle	1.491
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.040	0.570
Number of reflections	8738
<I/σ(I)>	21.9	2.3
Completeness [%]	99.0	100
Redundancy	6.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.2M NAF, 0.1M BISTRIS PROPANE PH8.5, 20% PEG3350, 10% ETHYLENE GLYCOL