2IWP
12th PDZ domain of Multiple PDZ Domain Protein MPDZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.710, 88.099, 73.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.830 - 2.150 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fne |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.830 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.090 | 0.470 |
| Number of reflections | 13731 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 50% PEG 300, 0.2M LI2SO4, 0.1M ACETATE PH 4.5 |
