2IVK
Crystal structure of the periplasmic endonuclease Vvn complexed with a 16-bp DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2005-12-17 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 64.636, 64.705, 79.157 |
Unit cell angles | 74.45, 73.56, 75.56 |
Refinement procedure
Resolution | 37.090 - 2.900 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oup |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.090 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.080 | 0.300 |
Number of reflections | 24597 | |
<I/σ(I)> | 17.5 | 3.3 |
Completeness [%] | 97.2 | 97.2 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% PEG3350, 0.2 M DI-SODIUM TARTRATE DEHYDRATE, pH 7.00 |