2IV3
Crystal structure of AviGT4, a glycosyltransferase involved in Avilamycin A biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.470, 74.370, 90.640 |
| Unit cell angles | 89.99, 92.70, 100.73 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iuy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.704 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.320 |
| Number of reflections | 56829 | |
| <I/σ(I)> | 9.8 | 2.6 |
| Completeness [%] | 96.9 | 92.3 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG 3350, 0.2M LI2SO4, 0.1M MES PH6.5 |
