2IUY
Crystal structure of AviGT4, a glycosyltransferase involved in Avilamycin A biosynthesis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-02-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.521, 73.829, 92.173 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.100 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.686 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.920 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.220 |
| Number of reflections | 42291 | |
| <I/σ(I)> | 22.8 | 5.5 |
| Completeness [%] | 97.9 | 86.9 |
| Redundancy | 6.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG3350, 0.2M LI2SO4, 0.1M MES PH 6.5 |
