2IUT

P. aeruginosa FtsK motor domain, dimeric

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	46.168, 57.431, 98.057
Unit cell angles	94.57, 93.80, 107.66

Refinement procedure

Resolution	100.000 - 2.250
R-factor	0.2207
Rwork	0.221
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.422
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.080	0.340
Number of reflections	39962
<I/σ(I)>	9.1	3.8
Completeness [%]	93.8	93.3
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1