2IUS

E. coli FtsK motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	97.600, 117.200, 132.800
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	100.000 - 2.700
R-factor	0.2459
Rwork	0.246
R-free	0.29830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.405
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.290
Number of reflections	80156
<I/σ(I)>	11.3	3.9
Completeness [%]	99.8	99.8
Redundancy	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1