2IUM
Structure of the C-terminal head domain of the avian adenovirus CELO long fibre (C2 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-25 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 216.836, 58.649, 57.591 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 16.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.21300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.618 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 88294 | |
| <I/σ(I)> | 20.9 | 2.3 |
| Completeness [%] | 93.8 | 73 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 10-12% PEG 10000; 0.1 M HEPES-HAOH PH 4.7-6.1; 0-10% GLYCEROL |
