2IUM
Structure of the C-terminal head domain of the avian adenovirus CELO long fibre (C2 crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-25 |
Detector | MARRESEARCH MAR165 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 216.836, 58.649, 57.591 |
Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
Resolution | 16.000 - 1.600 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.21300 |
Structure solution method | SIRAS |
RMSD bond length | 0.015 |
RMSD bond angle | 1.618 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.050 | 0.380 |
Number of reflections | 88294 | |
<I/σ(I)> | 20.9 | 2.3 |
Completeness [%] | 93.8 | 73 |
Redundancy | 3.1 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 10-12% PEG 10000; 0.1 M HEPES-HAOH PH 4.7-6.1; 0-10% GLYCEROL |