2IU8
Chlamydia trachomatis LpxD with 25mM UDPGlcNAc (Complex I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-07 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.805, 98.805, 283.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.209 |
Rwork | 0.206 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.344 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.610 |
Number of reflections | 65635 | |
<I/σ(I)> | 11.9 | 1.3 |
Completeness [%] | 91.1 | 95.1 |
Redundancy | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PROTEIN INCUBATED WITH 25MM UDPGLCNAC AND CRYSTALLIZED FROM 1.3M AMMONIUM SULFATE, 0.1M MES, PH 6.5, 2% PEG 400, AND 1MM TCEP. |