2IU6
REGULATION OF THE DHA OPERON OF LACTOCOCCUS LACTIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS-IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 101.090, 101.090, 148.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oi2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.662 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 59910 | |
| <I/σ(I)> | 22.6 | 6.88 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.38 | 10.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.15 M DL-MALIC ACID PH 7.0, 20% PEG-3350 |
