2IU4
Dihydroxyacetone kinase operon co-activator Dha-DhaQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.897, 99.897, 188.566 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.960 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.524 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.040 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.100 | 0.290 |
| Number of reflections | 77167 | |
| <I/σ(I)> | 11.17 | 5.66 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.39 | 4.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M SODIUM ACETATE TRIHYDRATE, PH 4.5, 2 M AMMONIUM SULFATE |
