2IU2
Recombinant human H ferritin, K86Q, E27D and E107D mutant, soaked with Zn ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-19 |
Detector | MARRESEARCH |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 181.050, 181.050, 181.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 104.260 - 1.800 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2chi |
RMSD bond length | 0.017 |
RMSD bond angle | 1.336 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.260 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.520 |
Number of reflections | 24095 | |
<I/σ(I)> | 28 | 6.6 |
Completeness [%] | 99.7 | 100 |
Redundancy | 18.6 | 18.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP. RESERVOIR : MPD 5.2%, CACL2 3.9 MM, TRIS 50 MM PH 8.5. DROP: 1UL PROTEIN (24 MG/ML) AND 1 UL RESERVOIR |