2IU2

Recombinant human H ferritin, K86Q, E27D and E107D mutant, soaked with Zn ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-19
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	181.050, 181.050, 181.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.260 - 1.800
R-factor	0.195
Rwork	0.193
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2chi
RMSD bond length	0.017
RMSD bond angle	1.336
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.260	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.520
Number of reflections	24095
<I/σ(I)>	28	6.6
Completeness [%]	99.7	100
Redundancy	18.6	18.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		HANGING DROP. RESERVOIR : MPD 5.2%, CACL2 3.9 MM, TRIS 50 MM PH 8.5. DROP: 1UL PROTEIN (24 MG/ML) AND 1 UL RESERVOIR