2ITL
The origin binding domain of the SV40 large T antigen bound to the functional pen palindrome DNA (23 bp)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9176 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.196, 64.096, 79.177 |
| Unit cell angles | 90.00, 120.69, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.650 |
| R-factor | 0.20378 |
| Rwork | 0.201 |
| R-free | 0.25061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ipr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 42961 | |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 20 mM di-ammonium hydrogen citrate, 20% PEG 3000, and 10 mM MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | di-ammonium hydrogen citrate | ||
| 2 | 1 | 1 | PEG 3000 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | di-ammonium hydrogen citrate | ||
| 5 | 1 | 2 | PEG 3000 | ||
| 6 | 1 | 2 | MgCl2 |
