2ITD
Potassium Channel KcsA-Fab complex in Barium Chloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-09-16 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 4 |
Unit cell lengths | 156.075, 156.075, 75.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.830 - 2.700 |
Rwork | 0.241 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.820 | 2.700 |
Rmerge | 0.074 | 0.044 | 0.490 |
Number of reflections | 28012 | ||
<I/σ(I)> | 12.9 | ||
Completeness [%] | 99.7 | 97.6 | 100 |
Redundancy | 8.3 | 7.9 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 21% PEG400, 50 mM MgAcetate, 5 mM BaCl2, 150 mM NaCl, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |