2ITD

Potassium Channel KcsA-Fab complex in Barium Chloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	200
Detector technology	CCD
Collection date	2005-09-16
Detector	ADSC QUANTUM 315
Spacegroup name	I 4
Unit cell lengths	156.075, 156.075, 75.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.830 - 2.700
Rwork	0.241
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.800
High resolution limit [Å]	2.700	5.820	2.700
Rmerge	0.074	0.044	0.490
Number of reflections	28012
<I/σ(I)>	12.9
Completeness [%]	99.7	97.6	100
Redundancy	8.3	7.9	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	21% PEG400, 50 mM MgAcetate, 5 mM BaCl2, 150 mM NaCl, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K