2IT6

Crystal Structure of DCSIGN-CRD with man2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	55.640, 55.640, 53.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.640 - 1.950
Rwork	0.196
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sl4
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.640	2.060
High resolution limit [Å]	1.950	1.950
Number of reflections	11951
<I/σ(I)>	7.3	3.2
Completeness [%]	99.9	100
Redundancy	4.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	Protein solution contained 10 mg ml-1 protein, 5 mM CaCl2, 25 mM Man2, reservoir solution contained 30% (w/v) polyethylene glycol 3000, 0.2 M NaCl, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K