2IT5

Crystal Structure of DCSIGN-CRD with man6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97945
Spacegroup name	P 43
Unit cell lengths	55.960, 55.960, 53.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.980 - 2.400
Rwork	0.198
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sl4
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.980	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	6501
<I/σ(I)>	6	3.2
Completeness [%]	99.6	99.8
Redundancy	4.6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	protein solution contained 5 mg ml-1 protein, 5 mM CaCl2, 50 mM Man6, reservoir solution contained 30% (w/v) polyethylene glycol 3000, 0.2 M NaCl, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K