2ISZ
Crystal structure of a two-domain IdeR-DNA complex crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.832, 69.739, 76.461 |
| Unit cell angles | 106.57, 104.85, 99.66 |
Refinement procedure
| Resolution | 50.000 - 2.403 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u8r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.134 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.075 | 0.043 | 0.296 |
| Number of reflections | 34892 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 90.4 | 98.5 | 60.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 28% PEG 3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | Bis-Tris | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | ammonium acetate | ||
| 6 | 1 | 2 | Bis-Tris |
