Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-02 |
| Detector | MARRESEARCH |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 397.360, 397.360, 397.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.227 * |
| Rwork | 0.227 |
| R-free | 0.28300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.290 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLOMON |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.100 | 2.800 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 | 0.300 |
| Total number of observations | 420859 * | |
| Number of reflections | 103157 | |
| <I/σ(I)> | 15.1 | 3.9 |
| Completeness [%] | 98.5 | 95.3 |
| Redundancy | 4.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 12 * | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium phosphate | 1.4 (M) | |
| 2 | 1 | drop | protein | 5 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 5 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | EGTA | 0.25 (mM) | |
| 6 | 1 | drop | DTT | 0.5 (mM) | |
| 7 | 1 | drop | sodium azide | 0.005 (%(w/v)) | |
| 8 | 1 | drop | CHAPSO | 0.05 (%(w/v)) | |
| 9 | 1 | drop | ammonium phosphate | 0.7 (M) |
