2ISB
Crystal structure of Fumarase of FUM-1 (NP_069927.1) from Archaeoglobus Fulgidus at 1.66 A resolution
Replaces: 1VPJExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000, 0.97966, 0.97950 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.664, 51.664, 157.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.152 - 1.660 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.19700 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.501 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 21.152 | 21.180 | 1.750 |
High resolution limit [Å] | 1.660 | 5.250 | 1.660 |
Rmerge | 0.088 | 0.054 | 0.664 |
Total number of observations | 5600 | 12686 | |
Number of reflections | 25660 | ||
<I/σ(I)> | 5.1 | 7.8 | 1 |
Completeness [%] | 97.8 | 97.8 | 86.2 |
Redundancy | 6.3 | 5.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 7.8 | 277 | 0.2M Li Acetate, 20.0% PEG-3350, No Buffer, pH 7.8, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |