2IRP

Crystal structure of the l-fuculose-1-phosphate aldolase (aq_1979) from aquifex aeolicus VF5

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-07-07
Detector	RIGAKU RAXIS V
Wavelength(s)	0.9788, 0.9793, 0.9000
Spacegroup name	I 4
Unit cell lengths	102.500, 102.500, 116.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.340 - 2.400
R-factor	0.2
Rwork	0.200
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.590
Number of reflections	22865
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	0.1M HEPES, 55% PEG 200, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K