2IQY
Rat Phosphatidylethanolamine-Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | SBC-3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.694, 70.588, 38.672 |
| Unit cell angles | 90.00, 109.96, 90.00 |
Refinement procedure
| Resolution | 26.230 - 1.400 |
| R-factor | 0.15321 |
| Rwork | 0.149 |
| R-free | 0.19299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.458 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.230 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.042 | 0.103 |
| Number of reflections | 28140 | |
| <I/σ(I)> | 13.6 | 7.45 |
| Completeness [%] | 89.3 | 48.2 |
| Redundancy | 3.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M Cacl2, HEPES pH 7.5 and 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
