2IQT
Crystal Structure of Fructose-Bisphosphate Aldolase, Class I from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | SBC-3 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.737, 68.737, 154.113 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.890 - 2.460 |
| R-factor | 0.18686 |
| Rwork | 0.182 |
| R-free | 0.23172 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.890 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.144 | 0.427 |
| Number of reflections | 16237 | |
| <I/σ(I)> | 7 | 3.8 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 8.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.2 M Ammonium citrate tribasic, 100 mM BTP, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
