2IQQ
The Crystal Structure of Iron, Sulfur-Dependent L-serine dehydratase from Legionella pneumophila subsp. pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.705, 77.151, 56.158 |
| Unit cell angles | 90.00, 117.91, 90.00 |
Refinement procedure
| Resolution | 38.580 - 2.660 |
| R-factor | 0.17957 |
| Rwork | 0.174 |
| R-free | 0.22590 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.736 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 2.760 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.104 | 0.665 |
| Number of reflections | 12986 | |
| <I/σ(I)> | 10.1 | 1.9 |
| Completeness [%] | 98.6 | 93 |
| Redundancy | 8.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50 mM Ammonium sulfate, 50 mM Bis-tris pH 6.5, 30 % Pentaerythritol ethoxylate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
