2IQ9
PFA2 FAB fragment, triclinic apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.564, 42.776, 58.355 |
| Unit cell angles | 95.81, 94.81, 91.11 |
Refinement procedure
| Resolution | 20.700 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ncw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16504 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 93.3 | 92.8 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 25% PEG3350, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, temperature 294K |
