2IQ6
Crystal Structure of the Aminopeptidase from Vibrio proteolyticus in Complexation with Leucyl-leucyl-leucine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-04 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 108.400, 108.400, 96.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cp6 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.500 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 23236 | |
<I/σ(I)> | 12.3 | 4 |
Completeness [%] | 96.2 | 78.3 |
Redundancy | 22.6 | 21.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | KSCN, NACL, TRIS, PH 8.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, TRIS REPLACED BY HEPES |