2IPZ
A Parallel Coiled-Coil Tetramer with Offset Helices
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.783, 53.878, 52.016 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 51.990 - 1.350 |
| R-factor | 0.20576 |
| Rwork | 0.202 |
| R-free | 0.23709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b22 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.047 | 0.395 |
| Number of reflections | 27103 | |
| <I/σ(I)> | 17.5 | 4.9 |
| Completeness [%] | 94.4 | 95.3 |
| Redundancy | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 295 | 10% Iso-propanol, 20% PEG 4000, 0.1M sodium bromide, 0.1M sodium HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
