2IPQ
Crystal structure of C-terminal domain of Salmonella Enterica protein STY4665, PFAM DUF1528
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.281, 55.039, 66.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
R-factor | 0.206 |
Rwork | 0.203 |
R-free | 0.27000 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.344 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.066 | 0.491 |
Number of reflections | 7182 | |
<I/σ(I)> | 10.1 | 1.5 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | (NH4)2SO4 0.2 M, Bis-Tris pH 6.5 0.1 M, PEG 3350 50 %, Vapor diffusion, Sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP |